🟤Microscopy Tutorials
Essential Microscopy Tutorials and Protocols
Essential Microscopy Tutorials and Protocols
Community-Curated by GerBI-GMB
Introduction
The GerBI-GMB (German BioImaging - Society for Microscopy and Image Analysis) community has curated a valuable collection of short educational videos and detailed protocols. These resources guide best practices in microscopy, helping users maintain, calibrate, and optimize their instruments for high-quality imaging. Below is a summary of key tutorials, direct access to each video, and accompanying protocols.
1. How to Measure a Point Spread Function (PSF)
Purpose: Determine the lateral and axial resolution of your microscope.
Protocol: QUAREP-LiMi Protocol for PSF Measurement
This video includes bead slide preparation and optimal acquisition settings. QUAREP-LiMi provides a detailed written protocol that includes image acquisition and analysis steps.
2. How to Clean Objective Lenses and Filters
Purpose: Maintain optical performance by safely removing dust and residues.
Learn to inspect and clean optical components without damaging their surfaces, ensuring sustained image clarity and protection of expensive parts.
3. How to Find the Focal Plane
Purpose: Accurately focus on the specimen without harming it.
This tutorial provides tips on achieving focus smoothly, minimizing risks to both sample and objective lenses.
4. How to Set Up Koehler Illumination
Purpose: Achieve even illumination across the specimen’s focal plane.
Understand the principle of Koehler illumination and follow a step-by-step alignment of lamp and condenser.
5. Live Cell Imaging and Environmental Control
Purpose: Ensure viability and reproducibility in live-cell imaging.
Explore strategies for maintaining temperature, humidity, and CO₂ levels, ranging from basic to advanced setups.
6. How to Minimize Photobleaching and Phototoxicity
Purpose: Protect live samples from light-induced damage during imaging.
Learn about minimizing light exposure and reactive oxygen species in fluorescence imaging experiments.
7. How to Calibrate a Camera
Purpose: Optimize camera performance through quantitative calibration.
This video walks you through:
Capturing dark images
Estimating readout noise and dark current
Generating flat field images
Measuring photon conversion factor and full well capacity
Disclaimer
This compilation is for educational and technical reference. Videos are hosted by iBiology and protocols are provided by QUAREP-LiMi. GerBI-GMB does not assume responsibility for implementation outcomes.
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